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Anti-Tubulin antibody produced in rabbit
Anti-Tubulin antibody produced in rabbit from MilliporeSigma
Be the first to write a review!
Citations:
(54)
Supplier Page
Supplier Page from
MilliporeSigma for
Anti-Tubulin antibody produced in rabbit
The
Anti-Tubulin antibody produced in rabbit
from
MilliporeSigma
is a Rabbit Polyclonal antibody to TUBA1B, TUBB2A, TUBB2B, and TUBB4B. This antibody recognizes Mouse, and Chicken/Bird antigen.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Tubulin antibody produced in rabbit
Company
MilliporeSigma
Price
Supplier Page
View Company Product Page
Catalog Number
T3526
Conjugate/Tag
unconjugated
Reactivity
mouse, chicken
Target
Tuba1b, Tubb2b, Tubb2c, Tubb2a
Host
rabbit
Antibody Type
primary antibodies
Applications
WB, IF-I
Clonality
polyclonal
NCBI Full Gene Name
tubulin, beta 2B class IIB, tubulin, beta 2A class IIA, tubulin, beta 4B class IVB, tubulin, alpha 1B
NCBI Gene Aliases
4930542G03Rik, 2410129E14Rik, M(beta)2, Tuba2, Tubb2, Tubb2c, brdp, Tubb2c1
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (54)
(1)
Somatic nuclear blebbing in Caenorhabditis elegans is not a feature of organismal aging but a potential indicator of germline proliferation in early adulthood
G3 Genes|Genomes|Genetics
April 11, 2023
Qiang Fan, Xue-Mei Li, Chao Zhai, Bin Li, Shang-Tong Li, Meng-Qiu Dong
loading. Blots were incubated with anti-H3(
1:5000
, Cat#1791, Abcam) or anti-tubulin (
1:5,000
dilution, Cat#
T3526
,
Sigma-Aldrich
). Quantification was carried out using ImageJ. Stress condition For heat stress, on
(2)
Survival of compromised adult sensory neurons involves macrovesicular formation
Cell Death Discovery
November 24, 2022
Anand Krishnan, Aparna Areti, Prashanth Komirishetty, Ambika Chandrasekhar, Chu Cheng, Douglas W. Zochodne
(
1:600
,
Sigma
Cat No.
T8328
) and rabbit monoclonal anti-βIII tubulin antibody (
1:600
,
Sigma
Cat No.
T3526
). Cells were incubated with the primary antibodies for 2 h and then washed with PBS. The corresponding
(3)
Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside
Science Advances
September 9, 2022
Hiroshi Inaba, Yurina Sueki, Muneyoshi Ichikawa, Arif Md. Rashedul Kabir, Takashi Iwasaki, Hideki Shigematsu, Akira Kakugo, Kazuki Sada, Tomoya Tsukazaki, Kazunori Matsuura
incubated for 5 min, followed by a wash with 20 μl of BRB80. Then, 5 μl of anti-tubulin antibody (73 mg/ml;
T3526
,
Sigma-Aldrich
) was passed through the flow cell and incubated for 5 min. After washing with 20 μl of
(4)
Insulin signaling regulates longevity through protein phosphorylation in Caenorhabditis elegans
Nature Communications
December 1, 2021
Wen-Jun Li et al.
probed with anti-GFP (
1:3000
dilution, Cat#
11814460001
,
Roche
) or anti-tubulin (
1:5000
dilution, Cat#
T3526
,
Sigma-Aldrich
) antibodies. Horseradish peroxidase-conjugated secondary antibodies (
1:10,000
dilution,
(5)
Oxidative stress transforms 3CLpro into an insoluble and more active form to promote SARS-CoV-2 replication
Redox biology
November 26, 2021
Du L, Xie Y, Zheng K, Wang N, Gao M, Yu T, Cao L, Shao Q, Zou Y, Xia W,
et al
.
mouse
antibody (
Proteintech
,
60004-1-Ig
),
Anti-Tubulin antibody produced in rabbit
(
Sigma-Aldrich
,
T3526
), Alpha tubulin monoclonal
mouse
antibody (
Proteintech
,
66031-1-Ig
),
goat
anti-
mouse
antibody (
LI-COR
,
(6)
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring
Immunity
November 9, 2021
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A,
et al
.
,
Cell Signaling Technology
,Cat#
3230
; RRID AB_2128522 Rabbit polyclonal anti-alphaTubulin,
Sigma Aldrich
,Cat#
T3526
; RRID AB_261659 "Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP",
ThermoFisher
,Cat#
31430
; RRID AB_228307 "Goat
(7)
RNA-binding protein Maca is crucial for gigantic male fertility factor gene expression, spermatogenesis, and male fertility, in Drosophila
PLOS Genetics
June 28, 2021
Li Zhu, Ryuya Fukunaga
as below. Rabbit anti-Maca (1/10000, generated in this study), rabbit anti-Tubulin (1/1000,
Sigma
,
T3526
),
mouse
anti-alpha-Tubulin (1/1500, Sigma, PLOS Genetics | https://doi.org/10.1371/journal.pgen.1009655
(8)
Increased hippocampal TrkA expression ameliorates cranial radiation‑induced neurogenesis impairment and cognitive deficit via PI3K/AKT signaling
Oncology Reports
September 28, 2020
Shengjun Ji, Haohao Wu, Xin Ding, Qingqing Chen, Xing Jin, Jinming Yu, Ming Yang
(
1:5,000
; cat. no.
ABS16
), anti-β-actin (
1:10,000
; cat. no.
AV40173
) and anti-tubulin (
1:10,000
; cat. no.
T3526
) were purchased from
Sigma-Aldrich
, Merck KGaA. Goat anti-mouse HRP (
1:10,000
; code no.
115-035-166
)
(9)
Function of Cathepsin K in the Central Nervous System of Male Mice is Independent of Its Role in the Thyroid Gland
Cellular and Molecular Neurobiology
July 1, 2020
Stephanie Dauth et al.
stripped and re-probed for -tubulin, using a rabbit anti--tubulin primary antibody (
Sigma-Aldrich
Cat#
T3526
RRID:AB_261659), and an HRP-conjugated
goat
anti-rabbit secondary antibody, as described above. Immunoreactions
(10)
Effect of microtubule immobilization by glutaraldehyde on kinesin-driven cargo transport
Polymer Journal
June 1, 2020
Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
mg/mL protein-A (P6031, Sigma-Aldrich, USA), followed by a 73 mg/mL anti-tubulin antibody solution (
T3526
,
Sigma-Aldrich
, USA), was applied to the flow cell. The flow cell was washed with solution A (~80 mM
(11)
Dracorhodin perchlorate inhibits osteoclastogenesis through repressing RANKL‐stimulated NFATc1 activity
Journal of Cellular and Molecular Medicine
March 1, 2020
Yuhao Liu et al.
Antimouse IgG‐FITC antibody (Cat#
F9137
), anti‐vinculin antibody (Cat#
V9264
), anti‐tubulin antibody (Cat#
T3526
) and recombinant M‐CSF (Cat#
M6518
) were commercially available from
Sigma‐Aldrich
. Recombinant GST‐rRANKL
(12)
Podocytes Produce and Secrete Functional Complement C3 and Complement Factor H
Frontiers in immunology
January 1, 2020
Mühlig AK, Keir LS, Abt JC, Heidelbach HS, Horton R, Welsh GI, Meyer-Schwesinger C, Licht C, Coward RJ, Fester L,
et al
.
number: ab24586), tubulin and laminin (both rabbit,
1:100
,
Sigma
, St. Louis, MO, USA, catalog numbers:
T3526
,
L9393
) were used. Nuclei were stained with 4, 6-Diamidine-2-phenylindole dihydrochloride (DAPI) (
1:10,000
,
(13)
Rspo2/Int7 regulates invasiveness and tumorigenic properties of mammary epithelial cells
Journal of Cellular Physiology
December 25, 2019
Malgorzata Klauzinska, Bolormaa Baljinnyam, Ahmed Raafat, Jaime Rodriguez-Canales, Luigi Strizzi, Yoshimi Endo Endo Greer, Jeffrey S. Rubin, Robert Callahan
H-108) antibodies were from
Santa Cruz Biotechnology
(Santa Cruz, CA). Rabbit anti-α-tubulin (
1:5,000
, #
T3526
) was from
Sigma–Aldrich
(St. Louis, MO).
Alexa Fluor® 594 phalloidin
(#
A12381
) was from
Life Technologies
(14)
Integrin-linked kinase controls retinal angiogenesis and is linked to Wnt signaling and exudative vitreoretinopathy
Nature communications
November 20, 2019
Park H, Yamamoto H, Mohn L, Ambühl L, Kanai K, Schmidt I, Kim KP, Fraccaroli A, Feil S, Junge HJ,
et al
.
1: 5,000
), anti-α-parvin (
Abcam
,
ab11336
,
1: 5000
), anti-ILK (Abcam, ab49979,
1: 5000
), anti-tubulin (
Sigma
,
T3526
,
1: 5000
), anti-FLAG (
Sigma
,
F7425
,
1: 5000
), anti-β-catenin (
Cell signaling
, #
8814
,
1:11,000
), anti-
AKT
(15)
DEAD-box RNA helicase Belle posttranscriptionally promotes gene expression in an ATPase activity-dependent manner
RNA (New York, N.Y.)
July 1, 2019
Liao SE, Kandasamy SK, Zhu L, Fukunaga R.
sources and dilutions of the primary antibodies were as below. Rabbit anti-Tubulin (1/1000,
Sigma
,
T3526
),
mouse
anti-α-tubulin (1/1500,
Sigma
,
T9026
), mouse anti-GFP (1/1000,
Pierce
,
MA515256
), mouse anti-HA
(16)
EZH2-DNMT1-mediated epigenetic silencing of miR-142-3p promotes metastasis through targeting ZEB2 in nasopharyngeal carcinoma
Cell death and differentiation
June 1, 2019
Li Y, He Q, Wen X, Hong X, Yang X, Tang X, Zhang P, Lei Y, Sun Y, Zhang J,
et al
.
Cell Signaling Technology
,
5246
),
GAPDH
(
1:5000
;
Abcam
,
ab128915
), and a-tubulin (
1:500
;
SigmaAldrich
, Ronkonkoma, NY, USA,
T3526
). Horseradish peroxidase-conjugated antibody was used as the secondary antibody (anti-
mouse
or anti-rabbit;
(17)
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin
The EMBO journal
January 3, 2019
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C,
et al
.
methanol, depending on the markers. The following antibodies were used: rabbit anti‐tubulin (
1:100
;
T3526
Sigma‐Aldrich
, Milan, Italy),
guinea pig
anti‐Bassoon
(
1:300
;
141004
,
Synaptic Systems
, Goettingen,
(18)
LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes
Nature communications
October 2, 2018
Zhu L, Kandasamy SK, Liao SE, Fukunaga R.
as below. Rabbit anti-MARF1 (1/5000, generated in this study), rabbit anti-Tubulin (1/1000,
Sigma
,
T3526
),
mouse
anti-alpha-Tubulin (1/1500,
Sigma
,
T9026
), mouse anti-GFP (1/1000,
Pierce
,
MA515256
), mouse
(19)
In vitro
study on reversal of ovarian cancer cell resistance to cisplatin by naringin via the nuclear factor-κB signaling pathway
Experimental and therapeutic medicine
March 1, 2018
Zhu H, Gao J, Wang L, Qian KJ, Cai LP.
h at room temperature. The
membrane
was then incubated with NF‑κ B, P‑gp and anti‑tubulin (cat. no.
T3526
;
Sigma‑Aldrich
; Merck KGaA, Darmstadt, Germany) primary antibodies overnight at 4˚C, and incubated with
(20)
Tubulin or Not Tubulin: Heading Toward Total Protein Staining as Loading Control in Western Blots
PROTEOMICS
October 1, 2017
Christian P. Moritz
Aldrich 199.2 1000 5 200 1.00 Amido black A8181-1EA Sigma Aldrich 137 500 5 100 1.37
Anti-Tubulin
antibody
T3526
-1ML
Sigma Aldrich
882 1 0.025 40 22.05 Anti-Actin antibody
A2066
-.2ML
Sigma Aldrich
390.5 0.2 0.05 4 97.63 HRP-coupled
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Reviews
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Anti-Tubulin antibody produced in rabbit from MilliporeSigma
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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